Agarose mini gel electrophoresis
アガロースminiゲル電気泳動
1% agarose gel in TAE
15ml for one mini gel
10xTAE (Tris-acetate EDTA)
|
|
final conc |
for 1l |
|
Tris |
0.4M |
48.4g |
|
Acetic Acid |
0.4M |
11.4ml |
|
0.5M EDTA(pH8.0) |
0.01M |
20ml |
|
dH20 |
|
|
EtBr (ethidium bromide)
stock solution 10mg/ml
final 0.1ug/ml